Journal:

Article Title: Production of Borreliacidal Antibody to Outer Surface Protein A In Vitro and Modulation by Interleukin-4

doi:

Figure Lengend Snippet: Borreliacidal antibody response of lymph node cells obtained from 17-day-vaccinated mice cultured with macrophages and B. burgdorferi and treated with anti-murine IL-4 10 min (checkered bar) or 4 days (cross-hatched bar) after cultivation. Control cultures (solid bar) were treated with a non-isotype-specific antibody. Titers of borreliacidal antibody did not vary by more than one dilution in replicate assays. In most cases (90%) the same titer was obtained.

Article Snippet: In other experiments, recombinant IL-4 (rIL-4) or rat anti-murine IL-4 (R&D Systems, Minneapolis, Minn.) at quantities ranging from 0.01 to 1.0 μg was added to cultures of immune lymph node cells, macrophage, and B. burgdorferi at 10 min and 4 days of incubation.

Techniques: Cell Culture

Journal:

Article Title: Calprotectin Expression by Gingival Epithelial Cells

doi: 10.1128/IAI.69.5.3248-3254.2001

Figure Lengend Snippet: Treatment of immortalized keratinocytes with IL-1β (0.1, 1, and 10 ng/ml) for 2 or 4 h. Immortalized keratinocytes were incubated with various concentrations of IL-1β (0.1, 1, and 10 ng/ml) for 2 h (black bars) or 4 h (crosshatched bars). Expression of mRNA for IL-8-, MRP8-, and MRP14-specific mRNA was determined by RNase protection assay. The signal intensity for the protected fragments was normalized to the abundance of the internal control GAPDH. The data shown are the mean ± the standard deviation (n = 3).

Article Snippet: Immortalized keratinocytes were stimulated by incubation with lipopolysaccharide from E. coli O55:B5 (Sigma, St. Louis, Mo.), phorbol 12-myristate 13-acetate (PMA; Sigma) or recombinant human IL-1β (R&D Systems, Inc., St. Paul, Minn.).

Techniques: Incubation, Expressing, Rnase Protection Assay, Standard Deviation